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KMID : 0364820080440020105
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Korean Journal of Microbiology
2008 Volume.44 No. 2 p.105 ~ p.109
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The Study on Function and Localization of Nup97 in Fission Yeast
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Hwang Duk-Kyung
Yoon Jin-Ho
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Abstract
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We studied on the function and localization of fission yeast Schizosaccharomyces pombe Nup97p, which is
homologous to nucleoporin Nic96p in budding yeast Saccharomyces cerevisiae. There was no effect on growth
and poly(A)+ RNA distribution of cells when nup97 gene was overexpressed. However, the haploid
Δnup97::kanr null mutants confirmed extensive poly(A)+ RNA accumulation in the nucleus, abnormal DNA
distribution, and cessation of growth when nup97 expression was repressed. We determined the subcellular
localization of Nup97 tagged at the N terminus or the C terminus with GFP. Both fusions complemented growth
defect of Δnup97::kanr null mutants. An integrated version of the nup97-GFP fusion was constructed at the
nup97 locus. Nup97-GFP fusions expressed from its own promoter was localized at the nuclear periphery with a
punctate appearance. These results suggest that Nup97p in fission yeast is also nucleoporin, which is involved in
mRNA export.
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KEYWORD
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mRNA export, nucleoporin, nup97, Schizosaccharomyces pombe
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